Introduction for antibody array
Antibody array (also known as antibody array) is a specific form of protein microarrays. In order to detect antigens, a collection of capture antibodies are spotted and fixed on a solid surface such as plastics. The function of antibody array is various. It is often used for detecting protein expression, stretching from cell lysates in general research and special biomarkers from serum or urine for diagnostic applications.
The history of antibody arrays
In 1983, Tse Wen Chang, who worked for Centocor, Inc. In Malvern, pennsylvania, found the concept and methodology of antibody arrays. On the purpose of detecting and quantifying the cells bearing certain surface antigens, such as, viruses and bacteria, and soluble antigens, Chang had invented this. He with another scientist set up a firm and bought the patents of antibody arrays from the former company. The first product was an assay which could can be used to monitor the immune status.
In 2000, the first use of antibody array for the protein-protein interaction and protein post-translational modification analysis in mammalian cells was reported. The first array approaches, which aimed to miniaturize biochemical and immunobiological assays performed in 96-well microtiter plates. 96-well antibody arrays were first created with 144 elements each for “standard enzyme-linked immunosorbent assays”. Similar arrays were used to measure prostate-specific antigen and cytokines.
The development of antibody arrays in recent years
At present, in the profiling experiments on tissue samples, plasma or serum samples and many other sample types, the antibody arrays are widely used. The main profiling studies for antibody array is cancer. In 2010, it was reported that the application of cancer-related research of antibody microarray comprising 810 different cancer-related antibodies.
The stretch knowledge of antibody array
We have mentioned that the antibody array is a specific form of protein array. The protein array, also known as protein chip, is a high-throughput method, which is used to track the interactions and activities of proteins, and also to determine the function and determining function on a large scale. The advantage of protein array is that it can track the large numbers of proteins in parallel. They are also rapid, automated, economical and highly sensitive, consuming small quantities of samples and reagents.
In a word, protein arrays and antibody arrays are the most important invention for today. Especially for the cancer-related researches, they have helped a lot in this field. To learn more detail knowledge about the two, you can find them on Wiki.